Product Name :
NQO1 Mouse Monoclonal Antibody [13A1]
Predicted band size :
31 kDa
Observed band size :
31 kDa
Synonyms:
Azoreductase antibody Cytochrome b 5 reductase antibody DHQU antibody DIA 4 antibody DIA4 antibody Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody Diaphorase (NADH/NADPH) antibody Diaphorase 4 antibody Dioxin inducible 1 antibody DT diaphorase antibody 展开
Function :
NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
Antibody Type:
Mouse Monoclonal Antibody
Immunogen :
Recombinant protein within Human NQO1 aa 1-274 / 274.
Species reactivity:
Human, Rat
Verify application:
WB
Coupling :
unconjugated
Clone number:
13A1
RRID:
AB_3068844
Form :
Liquid
Concentration :
2ug/ul
Storage instructions:
Store at +4℃ after thawing.1146118-59-3 structure Aliquot store at -20℃.6-Bromoquinolin-8-amine structure Avoid repeated freeze / thaw cycles.PMID:33437685
Storage buffer solution:
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG2b
Purification method :
Immunogen affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 31 kDa
Positive control :
A549 cell lysate, SiHa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HeLa cell lysate.