The manuscript, CO? supervised the experiments and suggested use of ELSD and discussed the outcomes, RHK supervised the whole project and revised the manuscript. Each of the authors study and authorized the final manuscript. Acknowledgment Egyptian Ministry of Larger Education and Beni-Suef University, Egypt are acknowledged for supporting YG. Author specifics 1 Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Salah Salem street, 62511 Beni-Suef, Egypt. 2Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Received: 4 March 2014 Accepted: 15 Could 2014 Published: 20 May 2014 References 1. Steckel H, Eskandar F, Witthohn K: The effect of formulation variables on the stability of nebulized aviscumine. Int J Pharm 2003, 257(1?):181?94. two. Naik A: Detergent formulations determined by soap and fatty acid N-methyl glucamide. 2007, WO Patent two,007,017,619. 3. Masyithah Z, Sembiring SB, Alfian Z, Herawan T: The optimization of enzymatic synthesis for lauroyl-n-methyl glucamide surfactants. Indonesian J Chem 2011, 11(three):223?28. four. Gaber Y, T nvall U, Orellana-Coca C, Ali Amin M, Hatti-Kaul R: Enzymatic synthesis of N-alkanoyl-N-methylglucamide surfactants: solvent-free production and environmental assessment. Green Chem 2010, 12(ten):1817?825. five. Maugard T, Remaud-Simeon M, Petre D, Monsan P: Enzymatic amidification for the synthesis of biodegradable surfactants: synthesis of N-acylated hydroxylated amines. J Mol Catal B Enzym 1998, five(1?):13?7. 6. Hill K, Rhode O: Sugar-based surfactants for consumer items and technical applications. Fett-Lipid 1999, 101(1):25?3. 7. Gonz ez S, Barcel?D, Petrovic M: Advanced liquid chromatography-mass spectrometry (LC-MS) techniques applied to wastewater removal and the fate of surfactants in the environment. Trends Anal Chem 2007, 26(2):116?24. eight. Eichhorn P, Knepper T: Fate research on the non-ionic surfactant alkyl glucamide by liquid chromatography/electrospray mass spectrometry. J Mass Spectrom 2000, 35(three):468?75. 9. Maugard T, Remaud-Simeon M, Petre D, Monsan P: Lipase-catalysed synthesis of biosurfactants by transacylation of N-methyl-glucamine and fatty-acid methyl esters. Tetrahedron 1997, 53(22):7629?634. 10. Gaber Y, T nvall U, Kumar MA, Ali Amin M, Hatti-Kaul R: HPLC-EAT (Environmental Assessment Tool): a tool for profiling safety, overall health and environmental impacts of liquid chromatography methods. Green Chem 2011, 13(eight):2021?025. 11. Wang Q, Zhang S, Yang J: HPLC analysis of sucrose ester analogs employing Evaporative Light Scattering Detection.Sodium difluoromethanesulfinate structure J Liq Chromatogr Relat Technol 2007, 30(16):2395?407.Buy6144-78-1 12.PMID:24957087 Aruda WO, Walfish S, Krull IS: Critique and optimization of linearity and precision in quantitative HPLC-ELSD with chemometrics. LC GC North Am 2008, 26(10):1032?042. 13. Orellana-Coca C, Adlercreutz D, Andersson MM, Mattiasson B, Hatti-Kaul R: Evaluation of fatty acid epoxidation by high efficiency liquid chromatography coupled with evaporative light scattering detection and mass spectrometry. Chem Phys Lipids 2005, 135(two):189?99.14. Park Hong S, Ryu Ho R, Rhee Choong K: Simultaneous separation of nine surfactants of various kinds by HPLC with evaporative light scattering detection. Talanta 2006, 70(three):481?84. 15. Maugard T, Remaud-Simeon M, Monsan P: Chemoselective amidification of amino-polyols catalyzed with lipases in organic solvents. Strategies Biotechnol 2001, 15:325?30.doi:ten.1186/1752-153X-8-33 Cite this article as: Gaber e.