, CCAGCTCGAGGGATTCAGGAATTGCTC CACCA; and reverse sequence, CCAGGCGGCCGCCTCCTCT GGCAGTAATGGTCCT. The primers were created
Chemexpress , CCAGCTCGAGGGATTCAGGAATTGCTC CACCA; and reverse sequence, CCAGGCGGCCGCCTCCTCT GGCAGTAATGGTCCT. The primers had been designed to consist of XhoI and NotI restriction web sites and a 4-bp extra random sequence. The PCR…